Biochemistry and Molecular Biology

Metabolic Pathways and Regulation Practice Questions

19 free Metabolic Pathways and Regulation practice questions for the USMLE Step 1, each with the correct answer and a detailed explanation. Open any question below, or take the full set as an interactive quiz.

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All Metabolic Pathways and Regulation questions

19 questions
  1. Q1. Which enzyme catalyzes the rate-limiting step of glycolysis and is strongly regulated by cellular energy status?
  2. Q2. What effect does high intracellular ATP concentration have on PFK-1 activity?
  3. Q3. Which metabolite can activate PFK-1 even in the presence of high ATP, thereby promoting glycolysis?
  4. Q4. Which three enzymes catalyze the essentially irreversible, regulatory steps of glycolysis?
  5. Q5. In the liver during fasting state, which hormonal signal leads to decreased glycolysis and increased gluconeogenesis?
  6. Q6. Which enzyme activity is inhibited by its own product, glucose-6-phosphate (feedback inhibition) to regulate glycolysis?
  7. Q7. Which enzyme in glycolysis is subject to feedforward activation by fructose-1,6-bisphosphate (F-1,6-BP)?
  8. Q8. Why is it important that glycolysis and gluconeogenesis are not active simultaneously in the same cell?
  9. Q9. Which enzyme regulates the reciprocal control between glycolysis and gluconeogenesis via modification of fructose-2,6-bisphosphate levels in liver?
  10. Q10. Which metabolic sensor kinase becomes activated when cellular AMP/ADP levels are high, promoting catabolic pathways and inhibiting anabolic pathways?
  11. Q11. In the citric acid (TCA) cycle, accumulation of which intermediate can allosterically inhibit phosphofructokinase-1 and thus slow glycolysis?
  12. Q12. Which statement best defines a branched metabolic pathway?
  13. Q13. Which enzyme initiates glycolysis in the liver by phosphorylating glucose when blood glucose is high, and has a higher Km for glucose than hexokinase?
  14. Q14. Which enzyme in gluconeogenesis is directly inhibited by fructose-2,6-bisphosphate, thereby preventing gluconeogenesis when glycolysis is active?
  15. Q15. Which of these is a catabolic pathway that generates reducing equivalents (NADH, FADH2) and feeds electrons into the electron transport chain?
  16. Q16. Which metabolic regulation mechanism refers to the end product of a pathway inhibiting an early enzyme in that same pathway?
  17. Q17. Which pathway provides reducing equivalents (NADPH) and ribose-5-phosphate for biosynthesis and antioxidant defense rather than ATP?
  18. Q18. During prolonged fasting, which enzyme is activated to promote gluconeogenesis from substrates like lactate or amino acids?
  19. Q19. Which enzyme in glycolysis is regulated both by allosteric effectors and by hormonal (covalent) modification in liver tissue?