Metabolic Pathways and Regulation · USMLE Step 1

Which enzyme activity is inhibited by its own product, glucose-6-phosphate (feedback inhibition) to regulate glycolysis?

  1. Glucokinase
  2. Hexokinase
  3. Phosphofructokinase-1
  4. Pyruvate kinase
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Correct answer: Hexokinase

Hexokinase is allosterically inhibited by its product, glucose-6-phosphate, preventing excessive phosphorylation of glucose when downstream metabolism is slowed, thus providing feedback regulation.

Difficulty: Medium Question 6 of 19

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