Metabolic Pathways and Regulation · USMLE Step 1

In the citric acid (TCA) cycle, accumulation of which intermediate can allosterically inhibit phosphofructokinase-1 and thus slow glycolysis?

  1. Oxaloacetate
  2. Isocitrate
  3. Citrate exported to the cytosol
  4. Succinyl-CoA
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Correct answer: Citrate exported to the cytosol

Citrate, exported from the mitochondria when TCA cycle intermediates accumulate, acts as an allosteric inhibitor of PFK-1 — signaling that energy and biosynthetic precursors are abundant and decreasing glycolytic flux.

Difficulty: Medium Question 11 of 19

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