Which enzyme catalyzes the rate-limiting step of glycolysis and is strongly regulated by cellular energy status?
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Correct answer: Phosphofructokinase-1 (PFK-1)
PFK-1 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and acts as the major regulatory checkpoint in glycolysis. Its activity is modulated by allosteric effectors like ATP, ADP/AMP and citrate, reflecting the energy status of the cell.
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More Metabolic Pathways and Regulation questions
- What effect does high intracellular ATP concentration have on PFK-1 activity?
- Which metabolite can activate PFK-1 even in the presence of high ATP, thereby promoting glycolysis?
- Which three enzymes catalyze the essentially irreversible, regulatory steps of glycolysis?
- In the liver during fasting state, which hormonal signal leads to decreased glycolysis and increased gluconeogenesis?
- Which enzyme activity is inhibited by its own product, glucose-6-phosphate (feedback inhibition) to regulate glycolysis?
- Which enzyme in glycolysis is subject to feedforward activation by fructose-1,6-bisphosphate (F-1,6-BP)?