Metabolic Pathways and Regulation · USMLE Step 1

Which enzyme initiates glycolysis in the liver by phosphorylating glucose when blood glucose is high, and has a higher Km for glucose than hexokinase?

  1. Glucose-6-phosphatase
  2. Glucokinase
  3. Phosphofructokinase-1
  4. Pyruvate kinase
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Correct answer: Glucokinase

Glucokinase, the hepatic isoform of hexokinase, activates only at high glucose concentrations and thereby acts as a glucose sensor, contributing to regulation of carbohydrate metabolism in the fed state.

Difficulty: Medium Question 13 of 19

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