Ultrastructure of cells · USMLE Step 1

What advantage does TEM (transmission electron microscopy) provide over light microscopy for studying cell ultrastructure?

  1. Ability to view live cell processes in real time
  2. True-color photographs of all cellular components
  3. Resolution far greater than light microscopy, down to ~0.1 nm
  4. Visualization limited to the external cell surface
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Correct answer: Resolution far greater than light microscopy, down to ~0.1 nm

TEM provides vastly higher resolution than light microscopy — on the order of tenths of a nanometer — allowing visualization of fine cell structures (membranes, organelles, ribosomes) not resolvable by light microscopy.

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