Molecular diagnostics · USMLE Step 1

Which molecular diagnostic method can provide information about both gene expression (mRNA levels) and possible gene mutations simultaneously in large number of genes?

  1. Western blotting of cellular proteins
  2. DNA microarray expression profiling
  3. FISH with chromosomal probes
  4. Sanger sequencing of a single gene
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Correct answer: DNA microarray expression profiling

DNA microarray-based expression profiling can measure mRNA levels of thousands of genes simultaneously and, depending on the array design, may also detect variant sequences — giving a broad overview of gene expression and potential mutations.

Difficulty: Medium Question 17 of 21

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