Molecular diagnostics · USMLE Step 1

Which of the following is a disadvantage of molecular diagnostic techniques compared to traditional culture or immunological assays?

  1. Generally lower analytical sensitivity
  2. Consistently longer assay turnaround time
  3. Need for specialized equipment and higher cost
  4. Inability to detect any genetic mutations
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Correct answer: Need for specialized equipment and higher cost

Molecular diagnostic methods often require specialized equipment (e.g., thermal cyclers, sequencers), trained personnel, and can be more expensive to set up than traditional assays, which can limit their availability in some settings.

Difficulty: Medium Question 19 of 21

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