Beta-Lactam Antibiotics · Pharmacology

What is the primary mechanism of action of beta-lactam antibiotics?

  1. Inhibition of protein synthesis at the 30S ribosomal subunit
  2. Binding penicillin-binding proteins to block cell wall synthesis
  3. Inhibition of bacterial DNA gyrase and topoisomerase IV
  4. Disruption of the outer membrane by binding to lipopolysaccharide
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Correct answer: Binding penicillin-binding proteins to block cell wall synthesis

Beta-lactams act as structural analogs of the D-Ala-D-Ala substrate. They covalently bind to PBPs, which are enzymes responsible for cross-linking peptidoglycan chains, leading to bacterial cell lysis.

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