Fixatives · Pathology

Which of the following fixatives is recommended for fixing lymph nodes to enhance nuclear detail for lymphoma diagnosis?

  1. B5 fixative
  2. Paraformaldehyde
  3. Normal saline
  4. Cold acetone
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Correct answer: B5 fixative

B5 fixative (containing mercuric chloride and formalin) was traditionally used for hematopoietic and lymphoid tissues because it provides crisp nuclear detail. Many labs now use zinc-formalin as a safer substitute.

Difficulty: Medium Question 10 of 20

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