Fixatives · Pathology

Which fixative is used both as a primary fixative for lipids and a secondary 'stain' for electron microscopy?

  1. Picric acid solution
  2. Osmium tetroxide
  3. Zinc sulfate fixative
  4. Absolute ethanol
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Correct answer: Osmium tetroxide

Osmium tetroxide chemically reacts with unsaturated fats, making them insoluble. It also provides high electron density, which gives contrast to membranes in electron microscopy.

Difficulty: Medium Question 12 of 20

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