Fixatives · Pathology

What is the primary drawback of using simple alcohol (Ethanol/Methanol) as a primary fixative for surgical specimens?

  1. It makes the tissue too soft for sectioning
  2. It causes severe shrinkage and excessive tissue hardening
  3. It is an additive, cross-linking fixative
  4. It promotes bacterial growth in the specimen
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Correct answer: It causes severe shrinkage and excessive tissue hardening

Alcohols are non-additive, coagulant fixatives that denature proteins by removing the water layer. While good for cytology, they cause extreme shrinkage and hardening of large tissue blocks.

Difficulty: Medium Question 15 of 20

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