The 'refractive index' of the mounting medium should be close to that of:
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Correct answer: Glass
To obtain the clearest image with minimal light distortion (refraction), the mounting medium and the coverslip glass should have similar refractive indices (usually around 1.51).
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More Tissue Preparation questions
- What is the effect of 'over-fixation' on a tissue sample?
- What is the primary objective of the 'fixation' step in tissue preparation?
- Which of the following chemical agents is the most commonly used primary fixative for light microscopy?
- Dehydration of a tissue specimen is typically achieved by passing it through a graded series of:
- What is the specific purpose of the 'clearing' step in the tissue processing sequence?
- During embedding, why must the tissue be carefully oriented in the mold?