Basic Histology Concepts

Tissue Preparation Practice Questions

20 free Tissue Preparation practice questions for the Histology, each with the correct answer and a detailed explanation. Open any question below, or take the full set as an interactive quiz.

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Questions

All Tissue Preparation questions

20 questions
  1. Q1. What is the primary objective of the 'fixation' step in tissue preparation?
  2. Q2. Which of the following chemical agents is the most commonly used primary fixative for light microscopy?
  3. Q3. Dehydration of a tissue specimen is typically achieved by passing it through a graded series of:
  4. Q4. What is the specific purpose of the 'clearing' step in the tissue processing sequence?
  5. Q5. During embedding, why must the tissue be carefully oriented in the mold?
  6. Q6. Which instrument is used to cut thin sections (typically 5–10 micrometers) from a paraffin-embedded block?
  7. Q7. What occurs during the 'infiltration' step of tissue processing?
  8. Q8. A 'frozen section' is often preferred over paraffin embedding in a clinical setting when:
  9. Q9. Which of the following is a disadvantage of using heat to speed up the fixation process?
  10. Q10. Before staining with an aqueous dye like Hematoxylin, what must be done to a paraffin-embedded section on a slide?
  11. Q11. What is the 'automated tissue processor' primarily used for?
  12. Q12. Which fixative is specifically recommended for preserving glycogen within tissues?
  13. Q13. Which of the following describes the 'mounting' step in histology?
  14. Q14. Why is 'Neutral Buffered' Formalin preferred over simple formaldehyde solutions?
  15. Q15. What is the purpose of the 'water bath' in the histology laboratory?
  16. Q16. Decalcification is a necessary step before processing which type of tissue?
  17. Q17. In the context of tissue preparation, 'grossing' refers to:
  18. Q18. Which reagent is most commonly used for clearing in the histology lab, despite being toxic?
  19. Q19. The 'refractive index' of the mounting medium should be close to that of:
  20. Q20. What is the effect of 'over-fixation' on a tissue sample?