Which reagent is most commonly used for clearing in the histology lab, despite being toxic?
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Correct answer: Xylene
Xylene is the most efficient clearing agent because it is highly miscible with alcohol and paraffin. However, it requires a fume hood due to its neurotoxicity and flammable nature.
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More Tissue Preparation questions
- The 'refractive index' of the mounting medium should be close to that of:
- What is the effect of 'over-fixation' on a tissue sample?
- What is the primary objective of the 'fixation' step in tissue preparation?
- Which of the following chemical agents is the most commonly used primary fixative for light microscopy?
- Dehydration of a tissue specimen is typically achieved by passing it through a graded series of:
- What is the specific purpose of the 'clearing' step in the tissue processing sequence?