During embedding, why must the tissue be carefully oriented in the mold?
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Correct answer: To enable the microtome to cut along the desired plane of section
Orientation determines the anatomical view (e.g., longitudinal vs. cross-section) seen under the microscope. Proper placement ensures that the diagnostically significant structures are exposed during microtomy.
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More Tissue Preparation questions
- Which instrument is used to cut thin sections (typically 5–10 micrometers) from a paraffin-embedded block?
- What occurs during the 'infiltration' step of tissue processing?
- A 'frozen section' is often preferred over paraffin embedding in a clinical setting when:
- Which of the following is a disadvantage of using heat to speed up the fixation process?
- Before staining with an aqueous dye like Hematoxylin, what must be done to a paraffin-embedded section on a slide?
- What is the 'automated tissue processor' primarily used for?