If an objective lens has a Numerical Aperture of 1.4, it is most likely designed for use with:
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Correct answer: Immersion oil
The maximum theoretical NA in air is 1.0 (since the refractive index of air is 1.0). Any NA significantly above 1.0 requires a medium with a higher refractive index, such as immersion oil ($n \approx 1.51$).
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More Microscopy Resolution questions
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