Microscopy Resolution · Histology

In the Abbe equation for resolution, $d = \frac{0.61 \lambda}{NA}$, what does 'lambda' ($\lambda$) represent?

  1. The wavelength of the light source
  2. The refractive index of the medium
  3. The numerical aperture of the lens
  4. The diameter of the objective lens
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Correct answer: The wavelength of the light source

Lambda represents the wavelength of the light used to illuminate the specimen. Shorter wavelengths result in a smaller value for 'd', which indicates higher (better) resolving power.

Difficulty: Medium Question 2 of 20

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