Microscopy Resolution · Histology

To resolve two objects that are 0.1 $\mu$m apart, which microscope must be used?

  1. Standard bright-field light microscope
  2. Phase-contrast microscope
  3. Stereo microscope
  4. Electron microscope
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Correct answer: Electron microscope

The limit of a standard light microscope is 0.2 $\mu$m. Since 0.1 $\mu$m is smaller than the wavelength limit of visible light, an electron microscope is required to visualize these objects as separate.

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