Histological Artifacts · Histology

Which artifact results in a 'shish-kebab' or 'telescoping' effect where tissue layers are pushed into one another?

  1. Improper grossing with a dull blade
  2. Over-staining with hematoxylin solution
  3. Fungal growth in the fixative
  4. Excessive heat in the processor
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Correct answer: Improper grossing with a dull blade

Using a dull blade during grossing or compression during the biopsy can cause tissue layers to collapse or telescope. This distorts the spatial relationship between different histological layers.

Difficulty: Medium Question 11 of 20

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