Histological Artifacts · Histology

Folds or wrinkles in a tissue section that cannot be removed on the water bath are often due to:

  1. The section being too thin
  2. The water bath being too hot
  3. Poorly processed, soft tissue
  4. Using a fresh microtome blade
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Correct answer: Poorly processed, soft tissue

If tissue is under-processed and soft, it lacks the structural integrity to flatten out on the water bath. The internal support is insufficient, causing the paraffin and tissue to compress and fold permanently.

Difficulty: Medium Question 17 of 20

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