What is the cause of 'out-of-focus' areas when viewing a slide that otherwise seems well-prepared?
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Correct answer: Trapped air or uneven mounting medium
Uneven mounting medium or air bubbles trapped beneath the coverslip create different focal planes. This makes it impossible to have the entire field of view in focus at the same time.
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More Histological Artifacts questions
- Which artifact is characterized by a blue haze or 'smudging' of the nuclear detail?
- Folds or wrinkles in a tissue section that cannot be removed on the water bath are often due to:
- Which of the following is a common 'contaminant artifact' seen as refractile, jagged crystals?
- The 'hole' seen in the middle of a tissue section after it is placed on a slide is often caused by:
- During H&E staining, if the nuclei appear brown instead of blue, the most likely cause is:
- Which of the following artifacts is most likely caused by improper dehydration or incomplete infiltration of paraffin during tissue process…