Histological Artifacts · Histology

A 'double contour' or 'ghosting' of nuclei is often an artifact related to:

  1. Rapid freezing and thawing
  2. Incomplete clearing
  3. Tissue lifting or poor slide adhesion
  4. Too much adhesive or coating on the slide
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Correct answer: Tissue lifting or poor slide adhesion

If the tissue section is not perfectly flat or begins to lift off the slide during staining, light refracts differently through the gaps. This can create a blurred or doubled appearance of cellular structures.

Difficulty: Medium Question 13 of 20

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