The 'hole' seen in the middle of a tissue section after it is placed on a slide is often caused by:
Show answer and explanation
Correct answer: Air trapped in the paraffin block
If air is trapped beneath the tissue during the embedding process, a void is created in the paraffin block. When sectioned, this void manifests as a hole or missing area in the center of the tissue section.
Keep practicing
More Histological Artifacts questions
- During H&E staining, if the nuclei appear brown instead of blue, the most likely cause is:
- Which of the following artifacts is most likely caused by improper dehydration or incomplete infiltration of paraffin during tissue process…
- What is the primary cause of 'chatter' or microscopic vibrations seen in a histological section?
- Which artifact appears as dark, needle-like brown crystals, often occurring when the pH of the fixative falls below 6.0?
- A 'venetian blind' appearance in a tissue section, characterized by parallel cracks or splits, is most frequently associated with:
- Which of the following describes 'crush artifact' commonly seen in small biopsies?