Transcription and RNA Processing · Zoology

Which of the following describes the function of the CTD (C-terminal domain) of RNA polymerase II?

  1. It acts as a helicase to unwind the DNA duplex
  2. It coordinates RNA processing by recruiting capping and splicing factors
  3. It binds directly to the TATA box sequence
  4. It catalyzes phosphodiester bond formation during elongation
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Correct answer: It coordinates RNA processing by recruiting capping and splicing factors

The CTD of RNA Pol II contains multiple heptapeptide repeats (YSPTSPS) that are progressively phosphorylated during transcription. Phosphorylated CTD acts as a scaffold to recruit 5' capping enzymes, splicing factors, and 3' processing machinery, coupling transcription with mRNA processing.

Difficulty: Medium Question 13 of 20

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