Transcription and RNA Processing · Zoology

What is the function of TFIIH in the eukaryotic transcription pre-initiation complex?

  1. It adds the 5' methyl cap to the nascent transcript
  2. It has helicase activity to unwind DNA and kinase activity to phosphorylate the CTD
  3. It recognizes and binds the TATA box sequence
  4. It catalyzes the formation and addition of the poly-A tail
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Correct answer: It has helicase activity to unwind DNA and kinase activity to phosphorylate the CTD

TFIIH is a multi-subunit general transcription factor. Its helicase activity creates the transcription bubble, while its kinase activity phosphorylates the RNA polymerase II CTD to trigger promoter clearance and the elongation phase.

Difficulty: Medium Question 18 of 20

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