Histopathology basics · USMLE Step 1

What is the primary advantage of combining special stains (e.g., PAS, trichrome, mucicarmine) with routine H&E when evaluating a tissue biopsy?

  1. Special stains replace the need for H&E altogether
  2. They reveal specific components—glycogen, mucin, collagen—poorly shown by H&E
  3. They are considerably faster to perform than H&E staining
  4. They can only be applied to frozen tissue sections
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Correct answer: They reveal specific components—glycogen, mucin, collagen—poorly shown by H&E

Special stains target biochemical or structural components (e.g., glycogen, mucins, collagen) that may not be differentiated clearly on H&E; thus, they complement H&E and enhance diagnostic accuracy, especially in pathologic conditions.

Difficulty: Medium Question 7 of 22

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