Which of the following sequences correctly describes the standard steps in preparing a tissue section for light-microscopy histopathology staining?
Show answer and explanation
Correct answer: Fixation → dehydration → embedding → sectioning → staining
Standard tissue processing involves fixation (to preserve tissues), dehydration (removing water), embedding (e.g., paraffin), sectioning into thin slices, then staining for microscopic evaluation. This order preserves morphology and allows proper staining.
Keep practicing
More Histopathology basics questions
- In an H&E-stained section, which of the following components would typically appear pink (eosinophilic)?
- Which of the following best defines the term Neoplasia in histopathology?
- Which special stain would be most helpful to highlight collagen fibers in a tissue biopsy?
- Which of the following is the most appropriate description of a malignant tumor (cancer) under histopathological criteria?
- Which of the following statements regarding the limitations of H&E stain is correct?
- Which of the following histopathological changes is most likely to be reversible if the injurious stimulus is removed?