Histopathology basics · USMLE Step 1

Which of the following statements regarding the limitations of H&E stain is correct?

  1. H&E can specifically identify mucin and glycogen reliably
  2. H&E provides excellent contrast for lipids and reticular fibers
  3. H&E poorly shows basement membranes, elastic fibers, and some pigments
  4. H&E cannot distinguish between nucleus and cytoplasm
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Correct answer: H&E poorly shows basement membranes, elastic fibers, and some pigments

While H&E is excellent for general tissue morphology, it poorly stains elements like basement membranes, elastic or reticular fibers, lipids, or certain pigments; these require special stains for accurate visualization.

Difficulty: Medium Question 13 of 22

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