Which technique would you choose to detect a known chromosomal translocation in leukemia cells?
Show answer and explanation
Correct answer: Fluorescence in situ hybridization (FISH)
FISH uses fluorescent DNA probes that bind to specific chromosomal regions; it can visualize rearrangements such as translocations directly in cells, making it ideal for detecting chromosomal translocations in leukemia.
Keep practicing
More Molecular diagnostics questions
- What is a key advantage of isothermal amplification (e.g., LAMP or RPA) over conventional PCR in resource-limited settings?
- Which molecular diagnostic technique is most suitable for confirming presence of a viral RNA pathogen in a patient sample?
- In molecular diagnostics, what does high analytical sensitivity mean?
- Which molecular diagnostic method can provide information about both gene expression (mRNA levels) and possible gene mutations simultaneous…
- What is the primary purpose of molecular diagnostics in oncology (cancer care)?
- Which of the following is a disadvantage of molecular diagnostic techniques compared to traditional culture or immunological assays?