Tetracyclines · Pharmacology

What is the primary mechanism of action of tetracyclines in inhibiting bacterial growth?

  1. Irreversible binding to the 50S ribosomal subunit to block peptide bond formation
  2. Inhibition of the DNA-dependent RNA polymerase enzyme
  3. Reversible binding to the 30S ribosomal subunit to block aminoacyl-tRNA entry
  4. Disruption of the cytoplasmic membrane integrity
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Correct answer: Reversible binding to the 30S ribosomal subunit to block aminoacyl-tRNA entry

Tetracyclines are bacteriostatic antibiotics that bind to the 30S subunit of the bacterial ribosome. This prevents the attachment of aminoacyl-tRNA to the A site, thereby halting protein synthesis.

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