Which component is uniquely required in the master mix of an RT-PCR reaction but not in a standard DNA-PCR?
Show answer and explanation
Correct answer: Reverse Transcriptase enzyme
Standard PCR begins with a DNA template and uses a DNA-dependent DNA polymerase. RT-PCR requires a Reverse Transcriptase enzyme to first convert the RNA template into cDNA before the DNA polymerase can function.
Keep practicing
More RT-PCR questions
- In molecular oncology, RT-PCR is frequently used to detect which of the following?
- What happens if the 'RNA Integrity Number' (RIN) of a sample is very low (e.g., < 2)?
- Which temperature is typically used for the annealing and activity of most commercial Reverse Transcriptases?
- Multiplex RT-PCR is used in clinical virology to:
- Why is the use of 'DEPC-treated water' important during the preparation of RT-PCR reagents?
- In RT-qPCR, what is the significance of the 'no-reverse transcriptase' (no-RT) control?