RT-PCR · Pathology

Which temperature is typically used for the annealing and activity of most commercial Reverse Transcriptases?

4°C - 10°C
42°C - 55°C
72°C - 75°C
94°C - 98°C

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Correct answer: 42°C - 55°C

Reverse transcription is generally performed between 42°C and 55°C. This range is optimal for the enzyme's activity while helping to destabilize some of the secondary structures (hairpins) in the RNA template.

Difficulty: Medium Question 13 of 20

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Which temperature is typically used for the annealing and activity of most commercial Reverse Transcriptases?

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