In molecular oncology, RT-PCR is frequently used to detect which of the following?
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Correct answer: Specific chromosomal fusion gene transcripts
Translocations result in fusion genes that produce unique mRNA transcripts. RT-PCR is highly sensitive for detecting these transcripts, such as the BCR-ABL1 fusion in Chronic Myeloid Leukemia, which is used for diagnosis and monitoring minimal residual disease.
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