In RT-qPCR, what is the significance of the 'no-reverse transcriptase' (no-RT) control?
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Correct answer: To identify contamination from genomic DNA
If a signal is detected in a control that lacks the reverse transcriptase enzyme, it indicates that the amplification is coming from contaminating genomic DNA rather than the cDNA. This control is vital for validating that the assay is specific to RNA.
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