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Molecular Pathology

RT-PCR Practice Questions

20 free RT-PCR practice questions for the Pathology, each with the correct answer and a detailed explanation. Open any question below, or take the full set as an interactive quiz.

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Questions

All RT-PCR questions

20 questions
  1. Q1. What is the primary function of the 'Reverse Transcriptase' enzyme in an RT-PCR reaction?
  2. Q2. In the context of RT-PCR, what is the specific purpose of using 'Oligo(dT)' primers during the reverse transcription step?
  3. Q3. Which of the following describes 'One-step' RT-PCR?
  4. Q4. What is the clinical significance of a 'One-step' RT-PCR assay in the detection of RNA viruses like SARS-CoV-2?
  5. Q5. When performing RT-PCR, why is it often necessary to treat the RNA sample with DNase I prior to reverse transcription?
  6. Q6. Which type of primer is most appropriate for reverse transcribing prokaryotic (bacterial) RNA or highly degraded RNA samples?
  7. Q7. In a Real-Time RT-PCR (RT-qPCR) assay, the term 'Relative Quantification' refers to:
  8. Q8. What is the role of 'RNase H' in the cDNA synthesis process?
  9. Q9. Which of the following represents a major disadvantage of 'Two-step' RT-PCR compared to 'One-step'?
  10. Q10. Which component is uniquely required in the master mix of an RT-PCR reaction but not in a standard DNA-PCR?
  11. Q11. In molecular oncology, RT-PCR is frequently used to detect which of the following?
  12. Q12. What happens if the 'RNA Integrity Number' (RIN) of a sample is very low (e.g., < 2)?
  13. Q13. Which temperature is typically used for the annealing and activity of most commercial Reverse Transcriptases?
  14. Q14. Multiplex RT-PCR is used in clinical virology to:
  15. Q15. Why is the use of 'DEPC-treated water' important during the preparation of RT-PCR reagents?
  16. Q16. In RT-qPCR, what is the significance of the 'no-reverse transcriptase' (no-RT) control?
  17. Q17. What is 'Nested RT-PCR' primarily used for?
  18. Q18. The 'Ct' value in RT-qPCR is inversely proportional to:
  19. Q19. Which of the following is a specific application of RT-PCR in the pathology of solid tumors?
  20. Q20. Which of these enzymes is 'thermostable' and used specifically in some one-step RT-PCR kits to allow higher RT temperatures?