In a Real-Time RT-PCR (RT-qPCR) assay, the term 'Relative Quantification' refers to:
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Correct answer: Normalizing target gene expression to a housekeeping gene
Relative quantification normalizes the expression of a target gene against a reference gene (like GAPDH or ACTB) that is expressed at a constant level. This accounts for variations in sample loading and RNA quality between different specimens.
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More RT-PCR questions
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- Which of the following represents a major disadvantage of 'Two-step' RT-PCR compared to 'One-step'?
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