Tissue Processing · Pathology

What is the primary purpose of 'fixation' in tissue processing?

  1. To remove water from the tissue
  2. To prevent autolysis and bacterial decomposition
  3. To make the tissue optically transparent for microscopy
  4. To soften the tissue for easier sectioning
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Correct answer: To prevent autolysis and bacterial decomposition

Fixation is the most critical step in tissue processing, aimed at preserving the biological morphology by denaturing proteins. This process halts enzymatic autolysis and prevents putrefaction by microorganisms.

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