Tissue Processing · Pathology

Which of the following describes 'Embedding' (or Blocking)?

  1. Replacing tissue water with ascending grades of alcohol
  2. Casting tissue into a wax mold to form a solid block
  3. Staining thin sections with Hematoxylin and Eosin dyes
  4. Cutting thin serial sections from the block using a microtome
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Correct answer: Casting tissue into a wax mold to form a solid block

Embedding is the process where the processed tissue is oriented in a mold and surrounded by molten wax, which then solidifies. This provides the necessary external support for the tissue to be sectioned on a microtome.

Difficulty: Medium Question 6 of 20

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