Tissue Processing · Pathology

What is the consequence of incomplete dehydration during tissue processing?

  1. The tissue will become too brittle and shatter
  2. The clearing agent cannot penetrate the tissue
  3. The tissue will be perfectly preserved for years
  4. The wax will infiltrate faster
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Correct answer: The clearing agent cannot penetrate the tissue

Wax is not miscible with water. If dehydration is incomplete, the clearing agent (like xylene) and the subsequent paraffin wax cannot properly penetrate the tissue, leading to soft, mushy centers that are impossible to section.

Difficulty: Medium Question 7 of 20

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