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Histopathology

Tissue Processing Practice Questions

20 free Tissue Processing practice questions for the Pathology, each with the correct answer and a detailed explanation. Open any question below, or take the full set as an interactive quiz.

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Questions

All Tissue Processing questions

20 questions
  1. Q1. What is the primary purpose of 'fixation' in tissue processing?
  2. Q2. Which of the following is the standard reagent used for the dehydration of tissues?
  3. Q3. Which step in tissue processing acts as the 'bridge' between dehydration and infiltration, using a reagent miscible with both alcohol and paraffin?
  4. Q4. What is the most common clearing agent used in routine histopathology laboratories?
  5. Q5. During infiltration, what is the typical temperature range for molten paraffin wax?
  6. Q6. Which of the following describes 'Embedding' (or Blocking)?
  7. Q7. What is the consequence of incomplete dehydration during tissue processing?
  8. Q8. Which chemical is commonly used as a 'mordant' in the context of some fixatives?
  9. Q9. What is 'Autolysis'?
  10. Q10. Which of the following fixatives is best suited for preserving lipids?
  11. Q11. What is the primary hazard associated with the use of Xylene in the lab?
  12. Q12. What is the recommended ratio of fixative volume to tissue volume for effective fixation?
  13. Q13. Which processing step is specifically required for specimens like bone or teeth before routine processing?
  14. Q14. What is the effect of 'over-fixation' in formalin?
  15. Q15. Which reagent is used as a 'secondary fixative' for electron microscopy to provide high contrast?
  16. Q16. What is the primary advantage of using a vacuum during tissue processing?
  17. Q17. Which of the following fixatives contains picric acid and is excellent for preserving glycogen but causes significant shrinkage?
  18. Q18. What is the 'refractive index' (RI) and why is it relevant in the clearing step?
  19. Q19. In a routine overnight processing schedule, which stage is usually the longest?
  20. Q20. Which of the following is a 'de-fatting' agent that can be used for very fatty tissues like breast?