Tissue Preparation · Histology

Which of the following describes the 'mounting' step in histology?

  1. Placing the tissue into the fixative
  2. Attaching and securing the paraffin block to the microtome chuck for sectioning
  3. Putting the slide into the microscope tray
  4. Applying a coverslip over the stained section with an adhesive medium
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Correct answer: Applying a coverslip over the stained section with an adhesive medium

Mounting is the final step where a drop of resinous medium is placed on the section and a glass coverslip is applied. This protects the tissue from damage and provides the correct refractive index for microscopy.

Difficulty: Medium Question 13 of 20

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