Microscopy Resolution · Histology

What is the relationship between Numerical Aperture (NA) and resolution?

  1. As NA increases, the minimum resolvable distance increases
  2. As NA increases, the resolving power decreases
  3. As NA increases, the minimum resolvable distance decreases
  4. NA has no effect on resolution, only on brightness
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Correct answer: As NA increases, the minimum resolvable distance decreases

Numerical Aperture is in the denominator of the resolution formula. A higher NA increases the light-gathering capacity, which results in a smaller minimum resolvable distance and superior image detail.

Difficulty: Medium Question 6 of 20

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