Which enzyme relieves the torsional strain and supercoiling created ahead of the replication fork?
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Correct answer: DNA Gyrase (Topoisomerase II)
As helicase unwinds the DNA, it creates positive supercoils ahead of the replication fork. DNA Gyrase (a type II topoisomerase) cuts, passes, and rejoins DNA strands to relieve this torsional tension, preventing the fork from stalling.
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