DNA Polymerase III requires a free 3'-OH group to begin synthesis. Which enzyme provides this by creating a short RNA sequence?
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Correct answer: Primase (RNA primer synthase)
Primase is a specialized RNA polymerase that synthesizes a short RNA primer (typically 5–10 nucleotides), providing the free 3'-OH group that DNA Polymerase III requires to begin extending the new DNA strand.
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