DNA Replication: Mechanisms and Enzymes · Zoology

What is the function of Single-Strand Binding Proteins (SSBs) during the replication process?

  1. To catalyze the formation of phosphodiester bonds
  2. To keep separated strands stable and prevent re-annealing
  3. To synthesize short RNA primers
  4. To proofread the newly synthesized DNA
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Correct answer: To keep separated strands stable and prevent re-annealing

Once helicase separates the DNA strands, single-strand binding proteins (SSBs) coat the single-stranded regions to prevent re-annealing or hairpin formation. This keeps the template accessible for replication machinery.

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