Laboratory Bio-Techniques · Zoology

Which of the following techniques separates molecules based on molecular size?

  1. Ion-exchange chromatography type
  2. Affinity chromatography methods
  3. Gel filtration chromatography
  4. Paper chromatography technique
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Correct answer: Gel filtration chromatography

Gel filtration chromatography (size-exclusion chromatography) separates molecules based on size as they pass through porous beads. Larger molecules cannot enter the pores and elute first, while smaller molecules enter the pores and take longer to pass through.

Difficulty: Medium Question 16 of 20

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