Laboratory Bio-Techniques · Zoology

The Rf value in chromatography represents:

  1. Rate of filtration speed
  2. Relative flow of solute
  3. Ratio of fragment length
  4. Reaction frequency value
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Correct answer: Relative flow of solute

The Rf (retardation factor) value is the ratio of the distance traveled by the solute to the distance traveled by the solvent front under the same conditions. It is a characteristic constant for a given substance in a specific solvent system and is used for compound identification.

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