Histopathology basics · USMLE Step 1

Which of the following special stains is best suited for detecting acidic mucopolysaccharides (glycosaminoglycans) in cartilage or mucinous tissue?

  1. Van Gieson stain
  2. Alcian blue stain
  3. H&E stain
  4. Oil red O stain
Show answer and explanation

Correct answer: Alcian blue stain

Alcian blue binds strongly to acidic polysaccharides such as glycosaminoglycans or mucopolysaccharides, rendering them bluish, and is commonly used to highlight cartilage matrix or mucin-rich tissues.

Difficulty: Medium Question 15 of 22

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