Histopathology basics · USMLE Step 1

Which of the following is considered a special histological stain used to identify mucin or glycoprotein in tissue sections?

  1. H&E stain
  2. Periodic acid–Schiff stain
  3. Van Gieson stain
  4. Toluidine blue stain
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Correct answer: Periodic acid–Schiff stain

The PAS stain highlights glycogen, mucins, mucoproteins, and glycoproteins in tissues, making it useful to detect mucin-producing cells or basement membranes. Special stains like PAS complement routine H&E when more biochemical detail is needed.

Difficulty: Medium Question 2 of 22

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